FIG. 1.

Identification of the malp gene sequence in M. fermentans strains. Genomic DNA from M. fermentans PG18 (lanes 1, 3, and 5) or strain II-29/1 (lanes 2, 4, and 6) was digested with EcoRI (lanes 1 and 2), NheI (lanes 3 and 4), or PstI (lanes 5 and 6), transferred to a nylon membrane, and hybridized with a denatured, 42-nt DIG-labeled malp probe. The probe was generated by PCR using degenerate oligonucleotides, based on the MALP-2 lipopeptide sequence, as described in Materials and Methods. Lane 7 contains DIG-labeled λ HindIII markers (Boehringer Mannheim), the sizes of which are indicated in kilobase pairs. The sizes of the single hybridizing fragments in each lane are 3 kb (lanes 1 and 2), 4.1 kb (lane 3), 2.7 kb (lane 4), 8 kb (lane 5), and 6.5 kb (lane 6).