FIG. 5.

Strain variation in the relative levels of MALP-2 and MALP-404. Total proteins from late-logarithmic-phase cultures of M. fermentans II-29/1 (lanes 1 to 5) or PG18 (lanes 7 to 9) were separated through a Tris-Tricine gel, transferred to a PVDF membrane, and immunostained with MAb F208C2B to the MALP-2 peptide sequence as described in Materials and Methods. Twofold dilutions of each protein sample were analyzed over a range that included equivalent immunostaining of the MALP-2 lipopeptide. The most concentrated sample from PG18 (lane 7) contained 10 μg of protein. Lane 6 contains low-molecular-weight markers (Gibco BRL), the sizes of which (in kilodaltons) are indicated on the left. The positions of the MALP-2 lipopeptide and the 41-kDa MALP-404 are indicated on the right. Lanes indicated by stars contain samples of the two strains with approximately the same amount of immunostained MALP-2.