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. 2022 Oct 24;12:1037934. doi: 10.3389/fonc.2022.1037934

Figure 2.

Figure 2

T7011 virus has no infectivity and cell killing activity on CAR T cells. (A, B) Viral yields recovered from T7011 or HSV-1 (F) infected CAR-TCD19 cells. CAR-TCD19 cells seeded in 12-well plates at a density of 5×105 cells per well were infected with or without HSV-1 (F) and T7011 at 0.1 PFU (A) or 1 PFU (B) per cell. The cell pellets were harvested at 2, 24, 48, and 72 h p.i. and lysed with three freeze-thaw cycles for titration to detect the virus titer. (C, D) CAR-TCD19 cells seeded in 96-well plates at a density of 4×104 cells per well were mock infected or infected with HSV-1 (F) and T7011 at 0.01, 0.1, or 1 PFU per cell for 48 h (C) and 72 h (D). The live cells were determined with CellTiter-Glo® as described in Methods and Materials. Results are expressed as the mean of the cell viability ± SEM compared to the mock infection control (as 100%). Statistically significant differences between HSV-1 (F) and T7011 infection groups are indicated by asterisks (**p < 0.01, ***p < 0.001).