Table 3.

Genome-wide DNA methylation analyses studies of mouse models of Alzheimer’s disease.

Study	Model	Brain tissue	Modification	Method	Main findings
Sanchez-Mut et al66	APP/PS1 (12 mo) 3xTg-AD (18 mo)	Twelve brain regions, including CA1, CA3 and DG hippocampal subregions, frontal cortex, and hypothalamus	5mC	MeDIP sequencing, Illumina VeraCode GoldenGate DNA Methylation Mouse Array	Hypermethylation of Sorbs3, Spnb4 and Tbxa2r in the prefrontal cortex of APP/PS1 and 3xTg-AD mice consistent with the hypermethylation of SORBS3, SPTBN4 and TBXA2R in post-mortem human AD brain tissue
Cong et al67	APP/PS1 (11 mo)	Cerebral cortex	5mC	MeDIP sequencing, Roche NimbleGen, Mouse DNA Methylation 3 ×720 K CpG Island Plus RefSeq Promoter Array	2346 hypermethylated regions of which 2221 mapped to promoter regions and 485 genes were associated with AD
Zhang et al68	SAMP8 (7 mo)	Cerebral cortex	5mC	WGBS	63 DMRs of which 41 regions were hypermethylated and 22 regions were hypomethylated in SAMP8 mice
Tang et al69	PSEN dKO (12 mo)	Hippocampus	5mC	RRBS	1094 hypermethylated regions and 1676 hypomethylated regions in PSEN dKO mice
Kundu et al70	APPNL-G-F and APPNL-F (12 mo)	Hippocampus	5mC	RRBS	57 DMRs shared between both AD mouse models
Shu et al48	APP/PS1 (12- and 67-wk)	Hippocampus	5hmC	hMeDIP sequencing	Decrease in total peaks in aged APP/PS1 mice
Zhang et al41	3xTg-AD (E16.5)	Cortical neurons (fetal brain)	5hmC	hMeDIP sequencing	Decrease in total peaks in AD group, with most 5hmC marks located in intergenic regions

Abbreviations: 5hmC, 5-hydroxymethylcytosine; 5mC, 5-methylcytosine; AD, Alzheimer’s disease; CA1, cornu ammonis 1; CA3, cornu ammonis 3; DG, dentate gyrus; dKO, double knock-out; DMR, differentially methylated region; MeDIP, methylated DNA-immunoprecipitation; WGBS, whole genome bisulfite sequencing ; RRBS, reduced representation bisulfite sequencing; hMeDIP, hydroxymethylated DNA-immunoprecipitation.