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. 2022 Oct 18;42(13):1359–1374. doi: 10.1177/03331024221110111

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© International Headache Society 2022

This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Immunofluorescence staining for 5HT_1B and 5HT_1D receptors in the VTA^PBP. (a) 5HT_1B receptors in the VTA^PBP (green); (b) 5HT_1B receptors in the paraventricular nucleus of the hypothalamus (PVN), as positive control (green); (c) Negative control in the VTA^PBP, obtained by omitting the primary antibodies; (d) 5HT_1D receptors in the VTA^PBP (green) and (e) 5HT_1D receptors in the substantia nigra, compact part, dorsal tier (SNCD) (green), as a positive control; Receptors are colocalized with DAPI (blue, nuclear marker). Arrows indicate examples in each image. Scale bars, 20 μm.