FIG. 2.

HPLC separation of [³H]AA metabolites released from S49 WT and S49 cyc⁻ cells exposed to native CT. After extraction of eicosanoids from the culture supernatants of three cultures, samples were chromatographed through a C₁₈ reverse-phase column, using an isocratic gradient of 27% acetonitrile in 0.1% TFA. All fractions were evaporated to dryness in a vacuum centrifuge and hydrated with 50% acetonitrile, and their levels of radioactivity were then measured in a liquid scintillation counter. The positions of selected PGs are indicated on the profiles.