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. 2022 Oct 20;13:1033208. doi: 10.3389/fendo.2022.1033208

Figure 4.

Figure 4

Impact of IGF1R deficiency on perigonadal adipose tissue and liver in young animals. (A) Effects of IGF1R depletion on perigonadal fat weight in 13 weeks-old CreERT2 mice. (B) Representative H&E staining of epididymal (males) and periovarian (females) fat depots. Scale bar, 100 µm. (C) Perigonadal fatty acid synthase (Fas) mRNA levels. Gene expression is expressed as fold changes (2-ΔΔCt) compared to its Igf1rfl/fl corresponding controls, considered as 1. (D) Liver weight. (E) Liver triglyceride content. (F) Gamma-glutamyltransferase (ƴGT) circulating levels. (G) Liver uncoupling protein 2 (Ucp2) and carnitine palmitoyl-transferase 1α (Cpt1α) mRNA levels expressed as fold changes (2-ΔΔCt) and compared to their Igf1rfl/fl corresponding controls, considered as 1. Data are expressed as mean ± SEM of at least 4 animals per group (n= 4-14 mice per group). *p < 0.05, **p < 0.01 and ***p < 0.001 vs. Igf1rfl/fl corresponding controls.