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. 2022 Sep 20;23(11):e55099. doi: 10.15252/embr.202255099

Figure EV1. UNC13D inhibits signaling induced by cytoplasmic DNA, but not RNA.

Figure EV1

  • A–L
    Quantification of the relative expression of relevant proteins in Fig 1A–L. n = 3 biological replicates.
  • M–O
    UNC13D stable knockdown (KD) HT29 cells and their control cells were transfected with plasmid DNA (1 μg/ml) (M and O) or infected with SeV (N) for the indicated time. Protein levels were detected by immunoblotting with anti‐UNC13D, ‐p‐TBK1, ‐p‐IRF3 or ‐GAPDH antibody (M and N). IFN‐β mRNA levels were measured by qPCR (O). n = 3 technical replicates.
  • P
    UNC13D knockout (KO) HeLa cells and their control cells were infected with GFP‐HSV for 18 or 24 h. GFP expression was examined via whole cell lysis (WCL) with immunoblotting (IB). The relevant band intensities were quantified using ImageJ. n = 3 biological replicates.

Data information: Statistically significant differences were determined using ANOVA. Data are shown as the mean ± s.d. ns, not significant, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.

Source data are available online for this figure.