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. 2022 Oct 10;23(11):e55251. doi: 10.15252/embr.202255251

Figure 6. Actin‐dependent nuclear positioning in 2‐cell embryos.

Figure 6

  • A
    Representative time‐lapse images of F‐actin (UtrCH‐mCherry) and nuclei (H2B‐mClover). Time is shown as h:min relative to anaphase onset. Arrows indicate high UtrCH‐mCherry intensity (n > 30 embryos).
  • B
    Representative time‐lapse imaging of F‐actin (UtrCH‐mCherry) after cytochalasin D addition (n = 10 blastomeres). Time is shown as h:min relative to drug addition. The look‐up table shows the colour scale corresponding to fluorescence intensity ranging from 0 to 100% of maximum.
  • C, D
    Representative time‐lapse imaging of F‐actin (UtrCH‐mCherry) after Jasplakinolide (C, n = 11 blastomeres) and SMIFH2 (D, n = 9 blastomeres) addition. Time is shown as h:min relative to drug addition. Arrows indicate increased UtrCH‐mCherry intensity relative to the last frame, and arrowheads indicate decreased UtrCH‐mCherry intensity.

Data information: In (A–D), data shown are representative examples of biological replicates as specified. Images are the maximum intensity projection of 3 z‐slices. Scale bars = 20 μm except in (B), scale bar = 10 μm.