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. 2022 Sep 14;23(11):e54993. doi: 10.15252/embr.202254993

Figure EV1. Induced transcription of ATG1 upon starvation is important for autophagy activity.

Figure EV1

  • A
    Atg1, Atg13, and Atg17 were HA‐tagged, respectively, at the C‐terminal by chromosome recombination and their expression levels were analyzed before and after nitrogen starvation in SD‐N medium.
  • B
    Atg1 was HA‐tagged at the C‐terminal by chromosome recombination in ypt7∆ cells and its protein levels were analyzed before and after nitrogen starvation in an SD‐N medium.
  • C–E
    Expression of exogenous Atg1 is controlled by the galactose promoter, and thus the expression levels of exogenous Atg1 in atg1∆ cells are controlled by different types of sugar added in the culture medium. Raffinose combined with galactose (2 and 0.01%, respectively) will induce low levels of Atg1 proteins while complete galactose (2%) will induce high levels of Atg1 proteins. Autophagic degradation of GFP‐Atg8 was measured.