Skip to main content
. 2022 Sep 14;23(11):e54993. doi: 10.15252/embr.202254993

Figure 4. The N‐terminal zinc figure domain and linker region of Rpb9 are important for its regulation on ATG1 transcription and autophagy.

Figure 4

  • A
    Schematic representation of yeast Rpb9 and indicated truncates checked for function in autophagy and regulation of ATG1 transcription.
  • B
    Indicated Rpb9 truncates and mutants were expressed in rpb9∆ cells and autophagic degradation of GFP‐50Q was checked by GFP‐processing assays.
  • C
    The truncates and mutants of the Rpb9 were expressed in rpb9∆ yeast cells and cell viability after 3‐day starvation in SD‐N medium was detected by serial dilution spotting and growth on YPD plates.
  • D
    Indicated Rpb9 truncates and mutants were expressed in rpb9∆ cells and ATG1 mRNA levels were analyzed by qRT‐PCR. Bars represent mean, error bars represent standard deviation, significance was determined by one‐way ANOVA (unpaired) followed by Tukey's multiple comparison test, *indicates P < 0.05, **indicates P < 0.01, ***indicates P < 0.001 (n = 5 biological replicates).