Figure EV4. Rpb9 alone cannot bind the ATG1 promoter.

• A
  Schematic diagram of Rpb9 in RNA polymerase II. The 10 subunits of yeast RNA polymerase II were shown as ribbon diagrams (this figure was prepared with PyMOL). Rpb9 was shown with orange color while the other subunits were shown in gray.
• B
  His‐tagged Rpb9 was purified from Escherichia coli cells and subject to an electrophoretic mobility shift assay (EMSA) together with DNA probes spanning the indicated promoter regions of ATG1.