Figure EV4. The effects of YTHDC1 knockdown on APA is a direct regulation from YTHDC1.

1. qRT‐PCR analysis of m⁶A related factors and 3′ end processing factors after knockdown of YTHDC1 in HEK293T cells. Knockdown of YTHDC1 has little effect on mRNA expression of those factors, which may mediate indirect effect on APA. Data are presented as mean ± SEM of three biological replicates. *P < 0.05, **P < 0.01, the P values were obtained from unpaired two‐tailed Student's t‐test.
2. Venn diagram of genes with differential expression levels and genes with APA sites switching after knockdown of YTHDC1 in HEK293T (left) and MCF7 (right). The siRNA‐APA denotes APA switching genes, siRNA‐DE denotes differential expression genes, and number denotes different siRNA. There are few intersections between genes with APA sites switching and genes with differential expression level, indicating that change of 3′UTR length was not caused by RNA degradation.