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. 2022 Oct 10;50(19):10839–10856. doi: 10.1093/nar/gkac864

Figure 4.

Figure 4.

A 20-nucleobase thyclotide enters cells and inhibits miR-21. (A) FACS of SKHEP1 cells either non-treated (blue) or treated with 5 μM of FAM-aegPNA 6 (orange) or FAM-thyclotide 7 (red). (B) FACS of HepG2 cells either non-treated (blue) or treated with 5 μM of either FAM-aegPNA 6 (orange) or FAM-thyclotide 7 (red). (C) Determination of miR-21 expression by RT-qPCR. HepG2 cells were treated with either 1 μM of scrambled thyclotide 8, antimiR-21 aegPNA 9 or antimiR-21 thyclotide 10, and apparent miR-21 levels were assessed and normalized to the average of miR-25 and miR-93 endogenous controls relative to the control scrambled expression.