Table 3.
Discrimination of single base mismatches and improvements in thyclotide. Tetrahydrofuran stereochemistry is (R,R). Tetrahydrofuran residues are represented by the symbol * in the sequences. PNA and thyclotide sequences are written from N- to C-terminal. All temperature values are reported in units of°C. ΔTm represents the difference in melting temperature between the complementary RNA and the RNA with the indicated mismatch. All mismatches were opposite to residue symbolized with T or T*
| Sequence | T m (ΔTm) (°C) | ||
|---|---|---|---|
| TU mismatch | TC mismatch | TG mismatch | |
| NH2-AGTCTGATAAGCTA-AEEA-CONH2 (1) | 62.0 (−8.6) | 60.5 (−10.1) | 63.4 (−7.2) |
| NH2-AGTCTGA*T*AAGCTA-AEEA-CONH2 (3) | 68.2 (−9.7) | 66.8 (−11.1) | 70.3 (−7.6) |