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. 2022 Oct 20;50(19):11058–11071. doi: 10.1093/nar/gkac913

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Rejoined episomal VDJ coding and signal joints in cells expressing K273A Lig4 have characteristics of a-EJ mediated joining. (A) Coding (left) and signal joints (middle) were PCR amplified from substrates isolated from cells of the indicated genotypes 72 h after transfection. (*) indicates a PCR artifact. Half of the PCR reaction for signal joint amplification was digested with ApaLI prior to electrophoresis (right). (B) Isolated coding joints from the indicated cell strains were subjected to amplicon sequencing. Histograms depicting numbers of nucleotides deleted or extent of microhomology at site of joining.