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. 2022 Oct 16;50(19):10857–10868. doi: 10.1093/nar/gkac897

Figure 3.

Figure 3.

The Gsyn:Ganti pair accommodates G489 in the minor groove. (A) Space filling representation of the Gsyn:Ganti pair and its location relative to G489 in the complex. (B) Space filling representation of U:A pair and its location relative to G489 in a complex with ADAR2 for the ideal 5′ nearest neighbor base pair (13). (C) (Top) Chemical structure of Gsyn:Ganti pair highlighting the location of the 2-amino group of the 5′ G relative to G489. (Bottom) Chemical structure of Ganti:Canti pair highlighting the location of the 2-amino group of the 5′ G relative to G489. (D) Overlay of ADAR2 R2D E488Q structures with RNA bearing either 5′ U paired with A (cyan) or 5′ G paired with G (salmon). Covalent hydration of 8-azanebularine, as seen by the out of plane oxygen present at the C6 position, enables trapping of these structures which mimic the adenosine deamination intermediate as confirmed in previous structures (13).