Figure 5.

In vitro deamination kinetics for ADAR1 p110 and duplex RNAs with nucleoside analogs paired with the 5′ G. (A) Sequence of model RNA substrate derived from the human IDUA mRNA as in Figure 1A. X = 2′-deoxyguanosine (dG), 2′-deoxyadenosine (dA) and 3-deaza-2′-deoxyadenosine (3-deaza dA) (B) Comparison of rate constants for reaction with 250 nM ADAR1 p110. See Table 5 for fitted values. Plotted values are the means of three technical replicates ± standard deviation. Statistical significance between groups was determined using one-way ANOVA with Tukey's multiple comparisons test; **P < 0.01; ***P < 0.001.