Fig. 2. Inhibitory effect of PEO on HT1080 cell adhesion: (a) HT1080 cells were pre-incubated in the absence (black bars) or in the presence (grey bars) of 40 μg mL⁻¹ of PEO for 30 min at room temperature. Cells were then added to 96-well microtiter plates coated with 10 μg mL⁻¹ fibronectin (Fn) or collagen I (Coll I) or with 50 μg fibrinogen (Fg) or poly-l-lysine (PL) 1 h at 37 °C. After washing, adherent cells were stained with crystal violet, solubilized by SDS and absorbance was measured at 600 nm. (b) Dose-effect of oil on HT1080 cancer cell adhesion to fibrinogen and fibronectin. Data shown are means ± SD of three experiments performed in triplicate and difference between treated and untreated (control) cells was considered statistically significant at *P < 0.05; **P < 0.01; ***P < 0.001.