Fig. 3. Inhibitory effect of PEO on HT1080 cell migration. (a) Cell motility was determined in a modified Boyden chamber. After treatment without or with 40 μg mL⁻¹ PEO for 30 min at room temperature, HT1080 cells were seeded into the upper reservoir and allowed to migrate. Metamorph imaging software was used to capture images of cell that migrate through the filter (scale bar: 100 μm). (b) Dose-effect of PEO on cell migration to matrix. Data shown are means ± SD of three experiments performed in triplicate and difference between treated and untreated (control) cells was considered statistically significant at *P < 0.05; **P < 0.01; ***P < 0.001.