Fig. 5. Selective inhibitory effect of PEO on HT1080 cell adhesion: HT1080 cells were preincubated without (black bars) or with 40 μg mL−1 PEO (grey bars). (a) Adhesion assays were performed with various cell/ECM protein pairs involving unique integrins: α1β1 (PC12/type IV collagen), α2β1 (HT1080/type I collagen), α5β1 (K562/fibronectin), αvβ3 (HT1080/fibrinogen), αvβ5 (HT29/vitronectin), α6β4 (HT29/laminin) and αvβ6 (HT29/fibronectin). (b) Adhesion was assessed using antibodies. (c) Dose–response curve of PEO induced inhibition of αvβ3, α5β1 and α2β1. Data shown are means ± SD of three experiments performed in triplicate and difference between treated and untreated (control) cells was considered statistically significant at *P < 0.05; **P < 0.01; ***P < 0.001.
