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. 1999 Feb;67(2):810–816. doi: 10.1128/iai.67.2.810-816.1999

FIG. 1.

FIG. 1

(A) Restriction enzyme digestion with XhoI and NcoI of the constructs pET20b(+)-CAT (lane 1) and pET20b(+)-GLU (lane 2). Molecular size standards in kilobases are indicated on the right. (B) Maps of the plasmids used for transformation of S. typhimurium BRD509 expressing the recombinant proteins CAT and GLU. The salmonella host was cotransformed with the plasmid pGP1-2, thus providing the temperature-inducible T7 RNA polymerase.