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Influence of cycloheximide on restoration of the initial ATP level in HEp-2 cells. (A) Control experiment. Cells were not treated with ShlA. (B to D) Initial ATP level depleted by ShlA to 30% (B) 25% (C), and 10% (D), followed by a change in the medium (to one with 5% FBS). □, no cycloheximide; ■, 2 μg of cycloheximide/ml. Similar results were obtained with HeLa cells. Values are means and standard deviations from experiments carried out three times in triplicate.
