TABLE 3.
ATP depletion in various cells induced by ShlA or viable S. marcescens strains
| Cell species | % of initial ATP level remaininga after incubation for 1 h at 37°C with:
|
||||
|---|---|---|---|---|---|
| ShlA
|
S. marcescensb
|
||||
| 1.5 HU/ml | 3 HU/ml | VA15854 | W1128 | CDC 04:H4 | |
| Granulocytes | 75 | 55 | NTc | NT | NT |
| Monocytes | 62 | 35 | NT | NT | NT |
| T cells | 85 | 80 | NT | NT | NT |
| Fibroblasts | 51 | 5 | NT | NT | NT |
| Endothelial cells | 100 | 100 | NT | NT | NT |
| Keratinocytes | 100 | 100 | NT | NT | NT |
| HeLa | 40 | 5 | 19 | 94 | 8 |
| HEp-2 | 50 | 14 | 32 | 100 | 28 |
| Hec1B | 12 | 0 | 22 | 100 | 15 |
| RT112 | 29 | 0 | NT | NT | NT |
| Chang | Lysed | Lysed | NT | NT | NT |
a
The values represent the means from three independent experiments performed in triplicate.
b
MOI, approximately 20; ShlA specific activity, 1.5 HU/μg.
c
NT, not tested.