Sequences and yields of glycosylated GLP-1 analogues synthesized by ApNGTQ469A and EndoCCN180H.
| Peptide ID | Peptide sequence (amino acids 7–37) | Glycosylation site | Yielda (%) | ||
|---|---|---|---|---|---|
| ApNGTQ469A | EndoCCN180H | ||||
| Glc | CT | SCT | |||
| 1 | HAEGTFTSN̲VSSYLEGQAAKEFIAWLVKGRG | 15N | 97.13 | 46.33 | 66.73 |
| 2 | HAEGTFTSDN̲SSYLEGQAAKEFIAWLVKGRG | 16N | N. D.b | — | — |
| 3 | HAEGTFTSDVSSYLEGQAAN̲ESIAWLVKGRG | 26N | 96.74 | 68.57 | 68.83 |
| 4 | HAEGTFTSDVSSYLEGQAAKEFIAWLVN̲GSG | 34N | N. D. | — | — |
| 5 | HAEGTFTSDVSSYLEGQAAKEFIAWLVN̲GTG | 34N | 94.44 | 63.49 | 61.19 |
a
Yields were determined by peak ratio of HPLC analysis of reaction mixtures.
b
N. D.: Not detected.