Fig. 1.

Experimental and sampling design. A Nutrient and light cycling conditions (yellow—on, gray—off) throughout the hours of the pulse-chase experiment. Samples were processed at 84 h. B Sample processing schematic for qPCR and subsequent stable isotope analyses. Host and symbiont tissues for each recruit were separated, and a subsample was used for quantitative analysis of each symbiont type. Using the relative ratio of symbiont types, groups 8–10 recruits were assigned, and host or symbiont tissues were combined into a glass fiber filter (GF/F)