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. 2022 Oct 19;13:1010470. doi: 10.3389/fpls.2022.1010470

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Copyright © 2022 Zhang, Chen, Anwar, Wang, Yao, Chen, Song and Su

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of the BES1 transcription factor that regulates BcGRP23 promoter activity. (A) Effects of different hormones on the expression of BcGRP23 in flowering Chinese cabbage. Different lowercase letters indicate significant differences at the p < 0.05 level. (B) Luciferase (LUC)/Renilla (REN) ratio indicating the relative luciferase activity in the dual-LUC assay. **Significant difference at the p < 0.01 level. (C, D) Yeast one-hybrid analysis. (C) Autoactivation of the BcGRP23 promoters was tested on synthetically defined (SD) medium without Ura in the presence of aureobasidin A (AbA). AbA¹⁰⁰ , 100 ng/ml AbA; AbA²⁰⁰ , 200 ng/ml AbA. (D) Interactions were determined on SD medium without Leu in the presence of 200 ng/ml AbA (−Leu+AbA²⁰⁰). (a) Negative control: AD-Empty+BcGRP23 promoter.