Figure 5.

Effect of LPS and 1G3E5 in CCL8 receptors’ expression in deer mice

(A) Expression of CCR1, 2, 3, 5, and 8 in the lungs of deer mice that received saline (n = 7), 1G3E5 at 2.5 mg/kg (n = 7), LPS (n = 8), and 1G3E5 (n = 8) at 2.5 mg/kg plus LPS. Expression was normalized versus GAPDH expression and expressed in arbitrary units (AU). Comparisons between all groups were performed by ANOVA, and the results are expressed as mean ± SEM. Significance vs ctr is indicated as follows: ∗, p < 0.05; ∗∗, p < 0.01, ∗∗∗∗, p < 0.00001.

(B and C) The heatmap correlation matrix depicts the degree of co-expression between chemokine receptors, CCL8, and pro-inflammatory cytokines in sibling pairs of deer mice that received LPS alone (B) or combined with 1G3E5 (C). Correlation coefficients are indicated.