TABLE 2.

E. coli plasmids used in this study

Plasmid	Description	Source or reference
Bluescript KS	Cloning vector	Stratagene
Hermes-8	E. coli-N. gonorrhoeae (ptetM25.2) shuttle vector	16
pC20	Promoterless cat gene in SspI site of opa-50 locus	17
pEMK55	opa-50 inserted in Hermes-6a	17
pTR25	pilC1; library clone	35
pTR30	pTR25; ermC in SmaI site	35
pTR32	pTR27; cat-1 between SmaI1 and SmaI3 sites	35
pTR36	pTR25; cat-1 in SmaI site	35
pTR45	pTR27; catlow between XhoI1 and XhoI2 sites	35
pTR81	pilC2iv in Bluescript KS	37
pTR102	pilC2iv in Hermes-8	35
pIS25	pilC2His6iv a in Bluescript KS	This work
pIS26	pilC2His6iv in Hermes-8	This work
pRH117	pPAH121; pilEF3	11

^apilC2_His6^iv, pilC2^iv with His6 coding sequence.