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. Author manuscript; available in PMC: 2022 Nov 7.
Published in final edited form as: DNA Repair (Amst). 2022 Sep 14;119:103405. doi: 10.1016/j.dnarep.2022.103405

Fig. 3.

Fig. 3.

Reconstituted MMR in vitro is not supported by the Mlh1(Q57L,Q59L)-Pms1 mutant. A. The DNA substrate used has a mispair disrupting a PstI site and a nick 442 bp 3′ from the mispair. Repair of this substrate requires Mlh1-Pms1 to make a nick 5′ of the mispair and is monitored by following the restoration of the PstI site using ScaI/PstI double digests. B. Example repair reactions showing robust repair in the presence of wild-type Mlh1-Pms1 complex and substantially reduced repair in the presence of Mlh1-Q57L,Q59L-Pms1 complex. C. Quantification of repair reactions shows that Mlh1(Q57L,Q59L)-Pms1 is unable to support MMR in vitro.