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. Author manuscript; available in PMC: 2022 Nov 7.
Published in final edited form as: DNA Repair (Amst). 2022 Sep 14;119:103405. doi: 10.1016/j.dnarep.2022.103405

Fig. 5.

Fig. 5.

Mlh1-Pms1 interface mutants are defective for Msh2-Msh6-mediated recruitment to mispaired DNA as measured by SPR. Binding of Msh2-Msh6 to an end-blocked DNA containing a mispair was monitored for the first 200 s. After this, flow was switched to buffer containing Msh2-Msh6 and the indicated concentrations of wild-type Mlh1-Pms1 (left), Mlh1-K54C-Pms1 (middle), or Mlh1-Q57L,T59L-Pms1 (right). Robust recruitment of wild-type Mlh1-Pms1 by Msh2-Msh6 is indicated by the rapid, concentration-dependent increase in response units observed. In contrast, the Mlh1-K54C-Pms1 and Mlh1-Q57L,T59L-Pms1 complexes show substantially or completely defective recruitment, respectively.