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. 2022 Oct 26;18(10):e1010463. doi: 10.1371/journal.pgen.1010463

Fig 2. TAP-tag proteomics.

Fig 2

A. TAP—Tandem Affinity Purification; TEV–Tobacco Etch Virus protease; Prot A–protein A; SBP–Streptavidin Binding Protein. Workflow of TAP-tag proteomics and Tandem MS followed by data elaboration (MaxQuant, Proteus) in combination with extended data mining by MIST, STRING, IPA, and g:Profiler. g:Profiler results were further refined and graphically elaborated in Cytoscape using the EnrichmentMap application. B. Volcano blot (Proteus) was created to distinguish DEK/NUP214 and empty vector interactomics—a synopsis of potential DEK/NUP214 interactors. C. MIST analysis of DEK/NUP214 interactors applying a threshold of Log2 fold change corresponding to an increase of interaction of 1.5-fold and a p<0.05.