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. Author manuscript; available in PMC: 2022 Nov 7.
Published in final edited form as: Prostate. 2019 Aug 21;79(14):1692–1704. doi: 10.1002/pros.23894

FIGURE 6.

FIGURE 6

Effect of WNT10B knockdown on EMT, metalloproteases and stemness gene expression and the stem-like cell population in PC3 cells. A, PC3 WNT10B shRNA cells showed an increased expression of E-cadherin and reduced expression of N-cadherin, vimentin, MMP9, NANOG, and SOX2 compared with control shRNA-PC3 cells. *P < .05 vs control cells, N = 3. Bars represent the mean ± SEM. B, Flow cytometry utilizing a Hoechst exclusion assay was used to assess effect of WNT10B knockdown in PC3 cells on the stem-like cell side population. Control shRNA and WNT10B shRNA-PC3 cells were stained with 5 μg/mL of Hoechst33342 either in the absence (left) or presence (right) of verapamil hydrochloride 15 minutes before fluorescence-activated cell sorting. The graph represents the percentage side-population cells (gated population) in control shRNA and WNT10B shRNA-PC3 cells. Knockdown of WNT10B significantly abrogated the stem-like cell side population. *P < .001 vs control cells, N = 4. Bars represent the mean ± SEM. EMT, epithelial to mesenchymal transition; shRNA, short hairpin