Figure 1. NaV1.1 is ubiquitously expressed in genetically identified proprioceptors.
(A–D) Representative confocal images of cryoprotected adult dorsal root ganglia (DRG) sections (25 μm) with pie chart quantifications indicating the percentage of Scn1a+ and Scn1a- neurons in each subpopulation (magenta and yellow, respectively). Images were acquired with a ×40, 0.9 NA water-immersion objective. Sections were hybridized using RNAscope with probes targeting Scn1a (Scn1a, magenta) and stained with the following antibodies (yellow): (A) anti-neurofilament heavy (NFH, n = 787), (B) anti-DsRed to label TdTomato+ proprioceptors (n = 153), (C) anti-calcitonin gene-related peptide (CGRP, n = 877), and (D) anti-GFP to label Mrgprd+ neurons (n = 744). DRG from C57BL/6, PvalbCre;Rosa26Ai14, and MrgprdGFP mice of both sexes were used. Scale bar 50 μm. White arrowheads indicate Scn1a+ neurons while white arrows indicate Scn1a- neurons. Frequency (E) and cumulative (F) distribution plots of integrated fluorescence density of the Scn1a signal in TdTomato+ proprioceptors (n = 153). n = cells.