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. Author manuscript; available in PMC: 2023 Jun 24.
Published in final edited form as: Circ Res. 2022 May 9;131(1):77–90. doi: 10.1161/CIRCRESAHA.121.320296

Figure 2. Selective targeting of miR-33 using pHLIP attenuates in vivo foam cell formation through induction of ABCA1.

Figure 2.

A, Representative images and flow cytometry histograms of in vivo foam cells from mice injected with PBS and fluorescently labeled pHLIP variant 3 constructs (A633-Var3). B, qRT-PCR analysis of miR-33 expression in peritoneal macrophages isolated from mice treated with anti-miR-33 peptide nucleic acid delivery vector (anti-miR-33pHLIP) or scrambled control (ScrpHLIP). Quantification represents the mean ± SEM (n=3). C, Representative Oil-red O (ORO) staining of in vivo foam cells from mice injected with anti-miR-33pHLIP or ScrpHLIP. Quantification of lipid accumulation are shown in the right panel and represents the mean ± SEM (n=5). D, Representative Western blot analysis of ABCA1 and ABCG1 in in vivo foam cells isolated from mice injected with anti-miR-33pHLIP or ScrpHLIP. Quantification is shown in the right panel and represents the mean ± SEM (n=5). Results were analyzed with a nonparametric Mann-Whitney test. Scale bar: 100 μm.