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. 2022 Aug 22;17(5):641–652. doi: 10.1016/j.ajps.2022.07.005

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© 2022 Shenyang Pharmaceutical University. Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig 7 — In vitro LDHA gene-editing of cancer cells by pCas9-sgLDHA/F3. (A) sgLDHA was designed to edit exon 4 of the LDHA gene. (B) The construction schemes of various plasmid DNA and expression products are illustrated. (C) B16F10 cells were treated with various lipoplexes, and genomic DNA was extracted for T7E1 assay. (D) B16F10 cells were treated with lipoplexes for 48 h, and the colors and pH values of culture media were assessed. (E) pCas9-sgLDHA/F3 mediated off-target gene editing was confirmed by T7E1 assay. Predicted top 2 potential off target sequence was suggested by Wellcome Sanger Institute Genome Editing (https://wge.stemcell.sanger.ac.uk//).