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. 2022 Nov 7;13(11):935. doi: 10.1038/s41419-022-05388-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Two SIRT2 siRNAs were transfected into the osteosarcoma cell lines MG63 and Saos-2, and Western blot was used to detect the knockdown efficiency of SIRT2. B A CCK-8 assay was used to detect cell viability in cells with SIRT2 knockdown for 4 or 8 consecutive days. C, D Transwell assays were employed to study the effect of SIRT2 knockdown (Si-2) on cell migration and invasion ability. Results from three experiments are summarized in a histogram format. E The photograph of the wound-healing experiment showed the migration distance of osteosarcoma cells to the scratch after SIRT2 knockdown (Si-2) at 0 h and 24 h. Results from three experiments are summarized in a histogram format. F The expression of SIRT2 was detected after transfection of the SIRT2 plasmid. G–I The CCK-8 assay, Transwell assay and wound-healing experiment were used to analyze SIRT2 overexpression on cell viability, migration and invasion. Results from three experiments are summarized in a histogram format. (*P < 0.05, **P < 0.01, ***P < 0.001).