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. 2022 Nov 7;13(11):935. doi: 10.1038/s41419-022-05388-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A The expression of EMT-related proteins was detected by Western blot after SIRT2 overexpression in U2OS cells. Results from three experiments are summarized in a histogram format. B The gelatin zymogram assay was used to detect the activity of MMPs with SIRT2 overexpression or knockdown. Data from a representative experiment of three independent samples are shown. C–E Representative immunofluorescence of N-cadherin, Vimentin and E-cadherin with SIRT2 overexpression was shown. The right panel was the summarized data of immunofluorescence score of related protein. (*P < 0.05, **P < 0.01, ***P < 0.001).