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. 2022 Nov 7;13(11):935. doi: 10.1038/s41419-022-05388-2

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Small animal in vivo imager was used to detect the metastasis of osteosarcoma cells in the SIRT2 knockdown group and the control group. B Fluorescence images of the lung and liver in the SIRT2 knockdown group and control group. A representative fluorescence image of at least three tissue samples is shown. The right panel is the summarized data of immunofluorescence density. C Photographs of the lung and liver of the two groups of nude mice. D, E H&E staining of paraffin-embedded lung and liver sections. The right panel is a magnification of the rectangle in the left panel, with arrows showing metastatic nodules. Number of metastatic nodules in the lung or liver is summarized in a histogram format. (*P < 0.05, **P < 0.01).