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. 2022 Nov 7;13(11):935. doi: 10.1038/s41419-022-05388-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A, B The pENTER-SIRT2-c-Flag-His and pcDNA3.1-Snail-c-HA plasmids were transfected into MG63 cells, and the binding of exogenous SIRT2 and Snail was detected by Co-IP. C Western blot was used to detect Snail expression in MG63 and Saos-2 cells. Data from a representative experiment of at least three independent samples are shown. D The expression of Snail was detected after SIRT2 knockdown in MG63 and Saos-2 cells. Data from a representative experiment of at least three independent samples are shown. E The expression of Snail was detected after SIRT2 overexpression in U2OS cells. F Immunohistochemical staining of SIRT2 and Snail was performed on the tumor tissues of the MG63-shSIRT2 group and control group. Immunohistochemical scores are summarized in a histogram format. G, H qRT-PCR was employed to detect Snail mRNA levels with SIRT2 knockdown or overexpression. (*P < 0.05, **P < 0.01, ***P < 0.001).