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. 2022 Nov 7;13(11):935. doi: 10.1038/s41419-022-05388-2

Fig. 8. Inhibition of SIRT2 activity downregulates Snail expression through the proteasomal degradation.

Fig. 8

A Treatment of MG63 cells with AGK2 showed a dose-dependent decrease in Snail expression. Data from a representative experiment of at least three independent samples are shown. B MG63 cells were treated with 30 μM AGK2 to detect the expression of Snail protein. C CHX was used to inhibit the synthesis of protein, and MG132 was used to treat MG63 cells. Data from a representative experiment of at least three independent samples are shown. D CHX and AGK2 were used to treat MG63 cells. Data from a representative experiment of at least three independent samples are shown. E After the combined treatment with MG132 and AGK2, Snail expression was detected. Data from a representative experiment of at least three independent samples are shown. F SIRT2-overexpressing cells were treated with AGK2 and Snail levels were detected. Data from a representative experiment of at least three independent samples are shown. G SIRT2-overexpressing cells were transfected with Snail siRNA and Snail levels were detected. Data from a representative experiment of at least three independent samples are shown. H, I Transwell and wound-healing assay were used to analyze the effect of Snail knockdown on the migration and invasion of SIRT2-overexpressing cells. Results from three experiments are summarized in a histogram format. (*P < 0.05, ***P < 0.001).