Skip to main content
. 2022 Nov 7;8:449. doi: 10.1038/s41420-022-01234-8

Fig. 5. LINC01468 destabilizes SHIP2 via ubiquitin proteasome pathway.

Fig. 5

A, B Western blotting analysis of SHIP2 expression levels in SNU-449 and HCC-LM3 cells after silencing LINC01468 (A) or in SNU-182 and Huh7 cells after overexpression of LINC01468 (B). C RT-qPCR was used to test the mRNA expression of SHIP2 in indicated cells transfected with (upper panel) or without (lower panel) LINC01468. GAPDH was used for normalization. D, E SNU-182 (D) and Huh7 (E) transfected with or without LINC01468 were treated with CHX at 20 μg/mL for the indicated times, and the expression of SHIP2 was tested by western blotting. F SNU-182 and Huh7 transfected with or without LINC01468 were treated with MG132 (5 μM for 4 h), and the SHIP2 protein level was tested by western blotting. G Quantification of the results in F. H The LINC01468-overexpressing SNU-182 cells were cotransfected with HA-tagged ubiquitin (HA-Ub) expressed plasmid. After MG132 (5 μM for 4 h) treatment, cell lysates were subjected to western blotting. Anti-HA antibody was used to perform IP. I The cell lysates in H were subjected to Co-IP with the SHIP2 (IP: SHIP2) antibody followed by western blotting. J Western blotting was used to detect cell lysates from Huh7 cells cotransfected with HA-Ub plasmid with or without LINC01468 and treated with MG132 (5 μM for 4 h). K The cell lysates in J were subjected to Co-IP with SHIP2 antibody (IP: SHIP2) followed by western blot analysis. *P < 0.05; **P < 0.01; ***P < 0.005.