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. 2022 Nov 7;8:449. doi: 10.1038/s41420-022-01234-8

Fig. 7. Enforced expression of SHIP2 rescues the LINC01468 metabolic phenotypes.

Fig. 7

A Western blotting analysis of the SHIP2/Akt/mTOR pathway protein in SNU-449 cells cotransfected with or without shLINC01468, together with shSHIP2 or empty vector. B Migration, invasion, ORO staining, and Nile Red staining were performed in SNU-449 cells as described in A. C Western blotting analysis of the SHIP2/Akt/mTOR pathway protein in Huh7 cells cotransfected with LINC01468 or the control, together with SHIP2 or empty vector. D The migration, invasion, ORO staining, and Nile red staining were performed in Huh7 cells as described in B. E, F Immunoblot detection of pmTOR level in SHIP2 E silencing in Huh7 cells or F overexpression in SNU-449 cells. G Immunoblotting of the indicated protein lysates from Huh7 cells expressing Ctrl or LINC01468 treated with or without the PIP3 inhibitor PIT-1. H Migration, invasion, ORO staining, and Nile Red staining were performed in SNU-449 cells as described in G. I Expression of LINC01468/SHIP2/mTOR cascade and Ki67 in HCC xenograft tissues. The expression of SHIP2, pmTOR, p-4E-BP1, and Ki67 was examined by IHC. Scale bars, 50 μm. Sof sorafenib, EVL everolimus (the mTOR inhibitor).