Fig. 1. Olaparib-treatment induces sister-chromatid exchanges in Brca2 wt and Brca2-mutant cancer cells.

A Representative Strand-seq libraries of Brca2^-/- cells or Brca2^iBAC cells, treated with DMSO (top) or olaparib (bottom). Black arrowheads indicate SCEs. B, C Brca2^-/- cells or Brca2^iBAC cells were treated with DMSO or olaparib. Quantification of SCEs (panel B) and CNVs (Panel C) per cell was done in n = 23 (Brca2^iBAC, DMSO), n = 26 (Brca2^iBAC, olaparib), n = 24 (Brca2^-/-, DMSO), and n = 19 (Brca2^-/-, olaparib) libraries. Means and standard deviations are indicated. D, E Brca2^-/- cells or Brca2^iBAC cells were treated with DMSO or olaparib, and SCEs were quantified by microscopy analysis of n = 20 metaphase spreads per condition (D). Scale bars indicate 10 μm. Averages and standard deviation are presented (E). For panels B, C, E statistics were performed using unpaired two-tailed t-tests (ns: non-significant). Gray bars indicate HR-proficient conditions, green bars indicate HR-defective conditions. Source data are provided with this paper.