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. 2022 Nov 7;13(11):932. doi: 10.1038/s41419-022-05390-8

Fig. 2. Knockdown of circFOXO3 on cell viability, apoptosis and ECM metabolism in ATDC5 chondrocytes.

Fig. 2

A The knockdown efficiency of circFOXO3 in ATDC5 chondrocytes was detected by RT-qPCR. (n = 4) ****p < 0.0001. B Alcian blue staining of siRNA-treated ATDC5 chondrocytes. C Cell viability determined by CCK-8 assay. (n = 3) ****p < 0.0001. D Western blot analysis of Cleaved PARP, Cleaved caspase-3, Bax, and Bcl2 when circFOXO3 was downregulated in ATDC5 chondrocytes. E The optical density analysis was performed from the results of three independent experiments of western blot samples. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. F Western blot analysis of MMP13, ADAMTS5 and Collagen II when circFOXO3 was downregulated in ATDC5 chondrocytes. G The optical density analysis was performed from the results of three independent experiments of western blot samples. *p < 0.05, **p < 0.01, ***p < 0.001. H, I ATDC5s proliferation activity was detected by TUNEL staining when circFOXO3 was downregulated. Representative photomicrographs and quantitative data showing the percentage of TUNEL-positive cells are shown. (n = 3) Scale bar, 200 µm. *p < 0.05, **p < 0.01. J, K Representative photomicrographs and fluorescence intensity of IF of MMP13, and Collagen II in ATDC5s after treating with IL-1β (10 ng/ml) for 24 h and the worsening effects of knockdown circFOXO3 on IL-1β. (n = 3) Scale bar, 25 µm. *p < 0.05, ***p < 0.001, ****p < 0.0001.