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. 2022 Nov 7;13(11):932. doi: 10.1038/s41419-022-05390-8

Fig. 3. Overexpression of circFOXO3 on cell viability, apoptosis and ECM metabolism in ATDC5 chondrocytes.

Fig. 3

A The overexpression efficiency of circFOXO3 in ATDC5s detected by qRT-PCR. (n = 4) ****p < 0.0001. B Alcian blue staining of lentivirus-treated ATDC5 chondrocytes. C Cell viability determined by CCK-8 assay. (n = 3) **p < 0.01, ****p < 0.0001. D, E Western blot analysis of Cleaved PARP, Cleaved caspase-3, Bax and Bcl2 when circFOXO3 was upregulated in ATDC5 chondrocytes. The optical density analysis was performed from the results of three independent experiments of western blot samples. *p < 0.05, **p < 0.01, ***p < 0.001. F, G Western blot analysis of MMP13, ADAMTS5 and Collagen II when circFOXO3 was upregulated in ATDC5 chondrocytes. The optical density analysis was performed from the results of three independent experiments of western blot samples. *p < 0.05, ***p < 0.001. H, I ATDC5s proliferation activity was detected by TUNEL staining when circFOXO3 was overexpressed. Representative photomicrographs and quantitative data showing the percentage of TUNEL-positive cells are shown. (n = 3) Scale bar, 200 µm. *p < 0.05, ***p < 0.001. J, K Representative photomicrographs and fluorescence intensity of IF of MMP13, and Collagen II in ATDC5s after treating with IL-1β (10 ng/ml) for 24 h and the saving effects of overexpressed circFOXO3 on IL-1β. (n = 3) Scale bar, 25 µm. *p < 0.05, ***p < 0.001.