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. 2022 Nov 7;13(11):932. doi: 10.1038/s41419-022-05390-8

Fig. 5. CircFOXO3 enhances autophagy in IL-1β-treated ATDC5 chondrocytes.

Fig. 5

A The expression levels of ATG5, Beclin1 and LC3 was detected by qRT-PCR when circFOXO3 was overexpressed in ATDC5s. (n = 3) **p < 0.01, ***p < 0.001, ****p < 0.0001. B The expression levels of ATG5, Beclin1 and LC3 was detected by qRT-PCR when circFOXO3 was downregulated in ATDC5s. (n = 3) **p < 0.01, ***p < 0.001, ****p < 0.0001. C, D Western blot analysis of LC3 and Beclin1 when circFOXO3 was overexpressed in ATDC5 chondrocytes. The optical density analysis was performed from the results of three independent experiments of western blot samples. ***p < 0.001, ****p < 0.0001. E, F Representative photomicrographs and fluorescence intensity of IF of LC3 in ATDC5s after treating with IL-1β (10 ng/ml) for 24 h and the saving effects of overexpressed circFOXO3 on IL-1β. (n = 3) Scale bar, 50 µm. ***p < 0.001, ****p < 0.0001. G Representative TEM images of chondrocytes treated with Lv-circFOXO3 or si-circFOXO3. Scale bars, 5 µm and 1 µm.