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. 2022 Nov 7;13(11):932. doi: 10.1038/s41419-022-05390-8

Fig. 6. CircFOXO3 autophagy sensitively regulates IL-1β-induced ATDC5 chondrocyte arthritis phenotype.

Fig. 6

A Representative Western blot of Beclin1 and LC3 in ATDC5 chondrocytes pretreated with Lv-circFOXO3 with or without IL-1β (10 ng/mL) in the presence or absence of CQ (50 μM) or 3-MA (5 mM) for 24 h. B quantitative analysis of the protein levels in Fig. 7B. (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. C Representative Western blot of Cleaved PARP, Cleaved caspase-3, Bax and Bcl2 in ATDC5 chondrocytes pretreated with Lv-circFOXO3 with or without IL-1β (10 ng/mL) in the presence or absence of CQ (50 μM) or 3-MA (5 mM) for 24 h. D quantitative analysis of the protein levels in Fig. 7D. (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. E Representative Western blot of MMP13, ADAMTS5 and Collagen II in ATDC5 chondrocytes pretreated with Lv-circFOXO3 with or without IL-1β (10 ng/mL) in the presence or absence of CQ (50 μM) or 3-MA (5 mM) for 24 h. F quantitative analysis of the protein levels in Fig. 7F. (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. G PI3K/AKT, Phosphorylation of PI3K/AKT and Beclin1, LC3 in ATDC5 chondrocytes infected with Lv-circFOXO3 with or without IL-1β (10 ng/mL) for different time span. H Quantitative analysis of the protein levels in Figure 8B. (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.