Figure 6.
Effects of GCM and GCM-KL -induced cytotoxicity of primary cortical mouse neurons. The primary embryonic culture of neurons was submitted to 24-h treatment with GCM or GCM-KL in different concentrations, 25% (A) and 50% (B). Cytotoxicity was assessed by the LDH assay. One-way ANOVA analysis, followed by Tukey's post-test, *p < 0.05, **p < 0.01. Results are presented as mean ± SEM of 5 independent experiments. (C) Representative schedule of the anti-inflammatory and neuroprotective effect of α-Klotho protein. LPS induces GCM to produce pro-inflammatory mediators that can lead to neuronal death (A). α-Klotho protein decreases the production of pro-inflammatory mediators induced by LPS in GCM (B), and it can have a protective effect on neurons from the neurotoxic effects of LPS induced by GCM. The figure was “Created with BioRender.com—Agreement number FH248VCXX8 to Scientific Reports”.