FIG. 2.

Naive (CD45RA⁺) and memory (CD45RO⁺) T cells respond to C. neoformans and CCW/M. Unseparated PBMC (□) or CD45RA (■)- or CD45RO (▨)-enriched cells were stimulated for 3 days (A) or 7 days (B). Irradiated AC (10⁵/well) were added to CD45RA- and CD45RO-enriched cells. The cells were stimulated with PHA (10 μg/ml), whole C. neoformans (2 × 10⁵/well), CCW/M (1.5 μg/ml), or tetanus toxoid. This experiment was repeated three times with similar, statistically significant results. ∗, P < 0.05 compared to the value for unstimulated PBMC; †, P < 0.05 compared to the value for unstimulated CD45RA-enriched cells; §, P < 0.01 compared to the value for unstimulated CD45RO-enriched cells; NS, not significant compared to the value for unstimulated CD45RA-enriched cells.